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Recombinant Anti-Estrogen Receptor alpha Mouse mAb (100 μl)
| Reactivity: | H & H |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Estrogen receptor |
Synonyms: | ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, ESR1, ESR, NR3A1 |
Immunogen: | Recombinant protein corresponding to Human ESR1 |
Uniprot ID: | P03372 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 66 kDa / 66 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human | 1: 500-1: 1000 | MCF7, T-47D |
IHC/IF | Human | 1: 500-1: 1500 | breast cancer |
Background
The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor.
Images
| WB analysis of Estrogen Receptor alpha(GB151845). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of Estrogen Receptor alpha(GB151845). Sample: Human breast cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of Estrogen Receptor alpha(GB151845). Sample: Human breast cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of Estrogen Receptor alpha(GB151845). Sample: Human breast cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
