Reactivity: | H,M,R & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | FARSLA rabbit polyclonal antibody |
Gene Name: | Phenylalanine--tRNA ligase alpha subunit |
Synonyms: | CML33, FARS, FARSA, FARSL, FARSLA, FRSA, PheHA, PheRS |
Immunogen: | Recombinant protein corresponding to Mouse FARSLA |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. | 58 kDa |
Observed MW. | 55 kDa |
Uniprot ID: | Q8C0C7 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | thymus, lung, testis |
IHC/IF | Mouse, Rat | 1: 1000-1: 3000 | hippocampus, cerebellum, brain |
Background
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression.
Images
Western blot analysis of FARSA (GB114111) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin embedded mouse hippocampus using FARSA (GB114111) at dilution of 1: 2000 | |
Immunohistochemistry analysis of paraffin embedded rat cerebellum using FARSA (GB114111) at dilution of 1: 2000 | |
Immunofluorescence of paraffin embedded mouse brain using FARSA (GB114111) at dilution of 1: 1000 | |
Immunofluorescence of paraffin embedded rat brain using FARSA (GB114111) at dilution of 1: 1000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |