Anti-FOXP3 Rabbit pAb (100 μl)

Forkhead box protein P3

Synonyms:

Scurfin, FOXP3, IPEX, JM2, AIID, DIETER, Immunodeficiency polyendocrinopathy enteropathy X linked, PIDX

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human FOXP3

Uniprot ID:

Q9BZS1

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Cytoplasm, Nucleus

Predicted MW. / Observed MW.

47 kDa / 47 kDa

WB

Human, Mouse, Rat

1: 500-1: 1000

lymph node, thymus

IHC/IF

Human, Mouse, Rat

1: 500-1: 1800

lymph node, thymus

WB analysis of FOXP3 (GB112325).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

IF analysis of FOXP3 (GB112325).
Sample: Mouse lymph node (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 900, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

IF analysis of FOXP3 (GB112325).
Sample: Rat thymus (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 900, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.