Your shopping cart is empty!
Recombinant Anti-Flag Tag Rabbit mAb (100 μl)
| Reactivity: | |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Gene Name: | Flag Tag |
Synonyms: | DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG |
Immunogen: | KLH conjugated Synthetic peptide corresponding to DYKDDDDK |
Isotype: | IgG |
Purity: | Affinity purification |
Product Usage Information
WB | Species independent | 1: 2000-1: 5000 | Transfected HEK-293F cells |
ICC/IF | Species independent | 1: 500-1: 1000 | Transfected HEK-293F cells |
Background
The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Images
| WB analysis of Flag Tag (GB15939). |
| ICC analysis of Flag Tag (GB15939) red. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
