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Recombinant Anti-GAPDH antibody (HRP Conjugated) (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | |
| Clonality: | |
| Gene Name: | Glyceraldehyde-3-phosphate dehydrogenase |
Synonyms: | GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
Conjugate | HRP |
Purity: | Affinity purification |
Uniprot ID: | P16858 |
Predicted MW. / Observed MW. | 36 kDa / 36 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1:1000-1:3000 | HeLa, HepG2, brain, etc. |
Incubation condition | After blocking, incubate the membrane with HRP conjugated anti-GAPDH antibody (ZB15004-HRP) for 2-3 hours at room temperature(20-25°C) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. In sperm, a testis-specific isoenzyme GAPDHS is expressed.
Images
| Western blot analysis of GAPDH (ZB15004-HRP) Sample: Protein treated by RIPA Lysis Buffer (G2002). |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
