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| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | GFM1 rabbit polyclonal antibody |
Gene Name: | Elongation factor G, mitochondrial |
Synonyms: | COXPD1, EF Gmt, EFG, EFG1, EFGM, EGF1, Elongation factor G1, GFM, GFM1, hEFG1, mEF G 1 |
Immunogen: | Recombinant protein corresponding to Mouse GFM1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. | 84 kDa |
Observed MW. | 84 kDa |
Uniprot ID: | Q8K0D5 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | pancreas, testis |
IHC/IF | Human, Mouse, Rat | 1: 800-1: 1600 | kidney |
Background
Different factors catalyze the three stages of protein translation: initiation, elongation, and termination. There are two translational systems in eukaryotes, one in the cytoplasm and the other in the mitochondria. In mitochondria, the elongation phase requires three elongation factors (EF): Tu (TUFM), Ts (TSFM), and G (GFM1)[PMID:19716793]. GFM1 catalyzes translocation during peptide elongation and mediates ribosomal disassembly during ribosome recycling in concert with the ribosomal recycling factor (RRF).
Images
 | Western blot analysis of GFM1 (GB114006) at dilution of 1: 1000 |
 | Immunohistochemistry of paraffin embedded human kidney using GFM1 (GB114006) at dilution of 1: 800 |
 | Immunohistochemistry of paraffin embedded mouse kidney using GFM1 (GB114006) at dilution of 1: 800 |
 | Immunohistochemistry of paraffin embedded rat kidney using GFM1 (GB114006) at dilution of 1: 800 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |