Recombinant Anti-GST Tag Rabbit mAb (100 μl)

Recombinant Anti-GST Tag Rabbit mAb (100 μl)

Brand: ServiceBio
Cat. #: GB15085-100
Availability: In Stock
$259.00
-+
Reactivity:     
Applications: WB    
Host Species: Rabbit    
Clonality: Monoclonal    
Gene Name:

GST  Tag

Synonyms:

Glutathione S-Transferase, GST, gst tag

Immunogen:

Recombinant GST protein

Isotype:

IgG

Purity:

Affinity purification

Predicted MW. / Observed MW.

26 kDa / 30 kDa




Product Usage Information

WB

Species independent

1: 1000-1: 3000

Transfected HEK-293F cells




Background

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.




Images

01 GB15085_GST Tag_重组H182_d50000_WB.png

WB analysis of GST Tag (GB15085). 

Sample: Protein treated by RIPA Lysis Buffer (G2002). 

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. 

Primary antibody: 1:3000, 4°C overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG(GB23303), 1: 3000, RT, 1h.




Storage

Storage            Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


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