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Anti-IL-1 beta Rabbit pAb (100 μl)
| Reactivity: | M,R & H |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | IL-1 beta rabbit polyclonal antibody |
Gene Name: | Interleukin-1 beta |
Synonyms: | IL1B, IL-1, IL1-BETA, IL1F2, interleukin 1 beta |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human IL-1β |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Lysosome, Secreted |
Predicted MW. | 31kDa |
Observed MW. | 35kDa |
Uniprot ID: | P01584, P10749, Q63264 |
Product Usage Information
WB | Human, Mouse | 1: 1000-1: 2000 | THP-1, Raw264.7 |
IHC/IF | Human, Mouse, Rat | 1: 800-1: 1600 | amygdalitis, lung+LPS, spleen+LPS |
Background
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
Images
| WB analysis of IL-1B (GB11113). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of IL-1β (GB11113). Sample: Human amygdalitis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of IL-1β (GB11113). Sample: Mouse lung+lps (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of IL-1β (GB11113). Sample: Mouse lung+lps (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of IL-1β (GB11113). Sample: Rat spleen+lps (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of IL-1β (GB11113). Sample: Human amygdalitis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IF analysis of IL-1β (GB11113). Sample: Mouse lung+lps (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| IF analysis of IL-1β (GB11113). Sample: Rat lung+lps (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
