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Recombinant Anti-KI67 Mouse mAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Proliferation marker protein Ki-67 |
Synonyms: | MKI67, marker of proliferation Ki-67, KIA,MIB-1, PPP1R105 |
Immunogen: | KLH conjugated Synthetic peptidecorresponding to Human Ki67 |
Uniprot ID: | P46013 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 359 kDa / 359 kDa |
Product Usage Information
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | colon cancer, colon |
Background
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
Images
| Immunohistochemistry analysis of paraffin embedded human colon cancer using KI67 (GB151142) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse colon using KI67 (GB151142) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded rat colon using KI67 (GB151142) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
