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| Reactivity: | H,M,R & H,M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Kinesin light chain 3 |
Synonyms: | kinesin light chain 2, kinesin light chain 3, KLC2, KLC2 like, KLC2L, KLC3, KLCt, KNS2B |
Immunogen: | Recombinant protein corresponding to Mouse KLC3 |
Uniprot ID: | Q91W40 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 56 kDa / 60 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 800-1: 1200 | testis |
ICC/IF | Human, Mouse | 1: 50-1: 100 | HeLa, NIH-3T3 |
Background
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
Images
| WB analysis of KLC3(GB114532). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of KLC3(GB114532)green. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 50, 4°C overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h. |
| ICC analysis of KLC3(GB114532)green. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 50, 4°C overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
