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Anti-Keap1 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Kelch-like ECH-associated protein 1 |
Synonyms: | keap1, INrf2, KLHL19, Kelch like ECH associated protein 1, KIAA0132, Cytosolic inhibitor of Nrf2 |
Immunogen: | Recombinant protein corresponding to Mouse Keap1 |
Uniprot ID: | Q9Z2X8 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 70 kDa / 60/64 kDa |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 300-1: 500 | kidney, colon |
IHC | Human | 1: 200-1: 400 | colon cancer, lung cancer |
Background
Keap1 has been shown to interact with Nrf2, a master regulator of the antioxidant response, which is important for the amelioration of oxidative stress. Under quiescent conditions, Nrf2 is anchored in the cytoplasm through binding to Keap1, which in turn facilitates the ubiquitination and subsequent proteolysis of Nrf2. Such sequestration and further degradation of Nrf2 in the cytoplasm are mechanisms for the repressive effects of Keap1 on Nrf2.
Images
| WB analysis of Keap1 (GB113747). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of keap1 (GB113747). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of keap1 (GB113747). Sample: Human lung cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
