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Anti-Ki67 Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | IHC/IF & ICC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Proliferation marker protein Ki-67 |
Synonyms: | MKI67, marker of proliferation Ki-67, KIA,MIB-1, PPP1R105 |
Gene ID: | 4288 |
Uniprot ID: | P46013 |
Immunogen: | KLH conjugated Synthetic peptidecorresponding to Human Ki67 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Product Usage Information
IHC/IF | Human, Mouse, Rat | 1: 300-1: 600 | esophageal cancer, ovarian cancer, spleen |
ICC/IF | Human, Mouse, Rat | 1: 100-1: 500 | HeLa |
Background
equired to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer and covers a substantial fraction of the chromosome surface . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility .
Images
| Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Ki67 (GB121141) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded human ovarian cancer using Ki67 (GB121141) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded mouse spleen using Ki67 (GB121141) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded mouse testis using Ki67 (GB121141) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded rat testis using Ki67 (GB121141) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded rat colon using Ki67 (GB121141) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed human colon cancer using Ki67 (GB121141) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed human liver cancer using Ki67 (GB121141) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse small intestine using Ki67 (GB121141) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat small intestine using Ki67 (GB121141) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using Ki67 (GB121141) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat testis using Ki67 (GB121141) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin embedded human ovarian cancer using Ki67 (GB121141) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded human colorectal carcinoma using Ki67 (GB121141) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded human esophageal carcinoma using Ki67 (GB121141) at dilution of 1: 500 |
| ICC analysis of Ki67(GB121141)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
| ICC analysis of Ki67(GB121141)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
