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Anti-LC3A/B Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | LC3A/B rabbit polyclonal antibody |
Gene Name: | Microtubule-associated proteins 1A/1B light chain 3 |
Synonyms: | LC3, LC3A, LC3A/B, LC3B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A, MAP1LC3A/B, MAP1LC3B |
Uniprot ID: | Q9GZQ8, Q9H492, Q62625, Q6XVN8, Q9CQV6, Q91VR7 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse LC3B |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 14/16 kDa |
Observed MW. | 14/16 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | brain, lung |
Background
LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.
Images
| Western blot analysis of LC3AB (GB11124) at dilution of 1: 1000 > |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
