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| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Lck rabbit polyclonal antibody |
Gene Name: | Proto-oncogene tyrosine-protein kinase LCK |
Synonyms: | Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK, Lck, Lsk-t |
Immunogen: | Recombinant protein corresponding to Mouse Lck |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cytoplasm |
Predicted MW. | 58 kDa |
Observed MW. | 58 kDa |
Uniprot ID: | P06239, P06240, Q01621 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | thymus, lymph node, spleen |
IHC/IF | Mouse, Rat | 1: 1500-1: 4000 | lung, lymph node, spleen, thymus |
Background
LCK is a member of the Src family of protein tyrosine kinases (PTKs). This protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules.
Images
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Western blot analysis of LCK (GB111091) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded mouse lung using LCK (GB111091) at dilution of 1: 1900 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse lymph node using LCK (GB111091) at dilution of 1: 1900 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse spleen using LCK (GB111091) at dilution of 1: 1900 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse thymus using LCK (GB111091) at dilution of 1: 1900 (400x lens) |
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Immunohistochemistry of paraffin embedded rat lymph node using LCK (GB111091) at dilution of 1: 1900 (400x lens) |
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Immunohistochemistry of paraffin embedded rat spleen using LCK (GB111091) at dilution of 1: 1900 (400x lens) |
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Immunohistochemistry of paraffin embedded rat thymus using LCK (GB111091) at dilution of 1: 1900 (400x lens) |
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Immunofluorescence of paraffin embedded mouse lung using LCK (GB111091) at dilution of 1: 1900 (400x lens) |
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Immunofluorescence of paraffin embedded mouse lymph node using LCK (GB111091) at dilution of 1: 1900 (400x lens) |
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Immunofluorescence of paraffin embedded mouse spleen using LCK (GB111091) at dilution of 1: 1900 (400x lens) |
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Immunofluorescence of paraffin embedded rat lung using LCK (GB111091) at dilution of 1: 1900 (400x lens) |
 | Immunofluorescence of paraffin embedded rat spleen using LCK (GB111091) at dilution of 1: 1900 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |