Recombinant Anti-MMP9 Mouse mAb (100 μl)

Matrix metalloproteinase-9

Synonyms:

MMP9, CLG4B, GELB, MANDP2, MMP-9, 92 kDa type IV collagenase, 92 kDa gelatinase, gelatinase B, matrix metallopeptidase 9

Immunogen:

Recombinant protein corresponding to Mouse MMP9

Uniprot ID:

P41245

Isotype:

IgG1

Purity:

Affinity purification

Subcellular location:

Secreted

Predicted MW. / Observed MW.

81 kDa / 92 kDa

WB

Mouse, Rat

1: 1000-1: 2000

Lung, spleen, spleen+LPS

IHC/IF

Mouse, Rat

1: 500-1: 2000

Inflammatory model induced by lps(6h) lung, lung injury

WB analysis of MMP9(GB15132).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h.

IHC analysis of MMP9(GB15132).
Sample: Mouse Inflammatory model induced by lps(6h) lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

IHC analysis of MMP9(GB15132).
Sample: Rat lung injury, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.