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Recombinant Anti-MUC1/CD227 Rabbit mAb (100 μl)
| Reactivity: | H |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Gene Name: | Mucin-1 |
Synonyms: | ADMCKD, ADMCKD1, CD227, MCD, MCKD, MCKD1, PEM, PUM, PEMT, Episialin, EAM, CA 15-3 |
Immunogen: | Synthesized peptide derived from human Epithelial Membrane Antigen(MUC1) |
Uniprot ID: | P15941 |
Isotype: | lgG |
Purity: | Affinity purification |
Subcellular location: | Secreted, Nucleus, Cell membrane,Cytoplasm |
Product Usage Information
IHC/IF | Human | 1: 500-1: 1000 | rectal cancer, kidney |
Background
The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.; The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response.
Images
| IHC analysis of MUC1 (GB154939). Sample: Human rectal cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2minBlocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of MUC1 (GB154939). Sample: Human kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2minBlocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
