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| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Nuclear export mediator factor Nemf |
Synonyms: | NEMF, NY CO 1, SDCCAG1, SDCG1 |
Immunogen: | Recombinant protein corresponding to Mouse NEMF |
Uniprot ID: | Q8CCP0 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 121 kDa / 17 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | heart, ovary |
IHC/IF | Mouse, Rat | 1: 2000-1: 4000 | testis |
Background
Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. NEMF is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety. Nemf is important for the stable association of Ltn1 to the complex. May indirectly play a role in nuclear export.
Images
| Western blot analysis of NEMF (GB114257) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse testis using NEMF (GB114257) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin embedded rat testis using NEMF (GB114257) at dilution of 1: 3000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
