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| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Deaminated glutathione amidase |
Synonyms: | NIT1, nitrilase 1, Nitrilase homolog 1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse NIT1 |
Uniprot ID: | Q8VDK1 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 36 kDa / 32 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 300-1: 800 | kidney, brain |
Background
Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Possesses amidase activity toward deaminated ophthalmate in vitro . Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.
Images
| Western blot analysis of NIT1 (GB114598) at dilution of 1: 800 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
