Reactivity: | M & H |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial |
Synonyms: | 3 oxoacid CoA transferase 1, OXCT, OXCT1, SCOT, SCOT s |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse OXCT1/SCOT |
Uniprot ID: | Q9D0K2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. / Observed MW. | 56 kDa / 50 kDa |
Product Usage Information
WB | Mouse | 1: 500-1: 1000 | heart |
IHC/IF | Human | 1: 400-1: 800 | kidney |
Background
Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
Images
Western blot analysis of OXCT1 (GB114721) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin embedded human kidney using OXCT1 (GB114721) at dilution of 1: 400 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |