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| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PCCA rabbit polyclonal antibody |
Gene Name: | Propionyl-CoA carboxylase alpha chain, mitochondrial |
Synonyms: | PCCA, PCCase subunit alpha |
Immunogen: | Recombinant protein corresponding to Mouse PCCA |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Mitochondrion matrix |
Predicted MW. | 80 kDa |
Observed MW. | 70 kDa |
Uniprot ID: | Q91ZA3 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | brain, kidney, liver |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | kidney |
Background
This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA.
Images
| Western blot analysis of PCCA (GB113958) at dilution of 1: 2000 |
 | Immunohistochemistry of paraffin embedded human kidney using PCCA (GB113958) at dilution of 1: 700 (200x lens) |
| Immunohistochemistry of paraffin embedded mouse kidney using PCCA (GB113958) at dilution of 1: 700 (400x lens) |
| Immunohistochemistry of paraffin embedded rat kidney using PCCA (GB113958) at dilution of 1: 700 (400x lens) |
| Immunofluorescence of paraffin embedded embedded human kidney using PCCA (GB113958) at dilution of 1: 500 (200x lens) |
| Immunofluorescence of paraffin embedded embedded mouse kidney using PCCA (GB113958) at dilution of 1: 500 (400x lens) |
 | Immunofluorescence of paraffin embedded embedded rat kidney using PCCA (GB113958) at dilution of 1: 500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
