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| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Histone lysine demethylase PHF8 demethylase PHF8) demethylase PHF8) |
Synonyms: | JHDM1F, KDM7B, KIAA1111, ZNF422 |
Immunogen: | Recombinant protein corresponding to Mouse PHF8 |
Uniprot ID: | Q80TJ7 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Product Usage Information
IHC/IF | Mouse, Rat | 1: 1000-1: 2000 | colon, testis |
Background
Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate.
Images
| Immunohistochemistry analysis of paraffin embedded mouse colon using PHF8 (GB114477) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse testis using PHF8 (GB114477) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded rat testis using PHF8 (GB114477) at dilution of 1: 1000 |
| Immunofluorescence analysis of paraffin embedded mouse testis using PHF8 (GB114477) at dilution of 1: 1000 |
| Immunofluorescence analysis of paraffin embedded rat testis using PHF8 (GB114477) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
