Reactivity: | H,M |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | Phosphorylase B/PHKB rabbit polyclonal antibody |
Gene Name: | Phosphorylase b kinase regulatory subunit beta |
Synonyms: | Phosphorylase kinase subunit beta, Phkb, FLJ41698, DKFZp781E15103 |
Immunogen: | Recombinant protein corresponding to Mouse Phosphorylase B/PHKB |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 124 kDa |
Observed MW. | 124 kDa |
Uniprot ID: | Q93100, Q7TSH2 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse | 1: 500-1: 1000 | skeletal muscle |
Background
PHKB gene encodes phosphorylase kinase subunit beta involved in glycan biosynthesis and glycogen metabolism. PHKB activity is regulated by phosphorylation of various serine residues, and catalyzes the phosphorylation of serine in certain substrates, including troponin I. Phosphorylase kinase (PhK) complex, composed of alpha, beta, gamma, and delta subunits, stimulates energy production from glycogen in the cascade activation of glycogenolysis. Its large homologous alpha and beta subunits regulate the activity of the catalytic gamma subunit.
Images
Western blot analysis of Phkb (GB111930) at dilution of 1: 500 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |