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| Reactivity: | H,M & H,M |
| Applications: | IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PKR rabbit polyclonal |
Gene Name: | Interferon-induced, double-stranded RNA-activated protein kinase |
Synonyms: | eIF-2A protein kinase 2, Interferon-inducible RNA-dependent protein kinase, Prkr, P1/eIF-2A protein kinase, Protein kinase R, Pkr, Eif2ak2, p68 kinase, Tik |
Immunogen: | Recombinant protein corresponding to Mouse PKR |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Human, Mouse | 1: 1000-1: 2000 | colon, brain, spleen, heart, pneumonia |
IF | Human, Mouse | 1: 500-1: 1000 | colon, brain, spleen, heart, pneumonia |
Background
PKR is an IFN-induced serine/threonine protein kinase consisting activated by dsRNA produced during viral infections, PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth and differentiation. Upon activation, PKR gets autophosphorylated and catalyzes the phosphorylation of elF2 alpha subunit, which leads to an inhibition of protein synthesis. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival and cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, and PDGF signaling pathway. PKR has been implicated in tumor suppression, malignancy, and several neurodegenerative diseases (Alzheimer, Huntington, and amyotrophic lateral sclerosis) .
Images
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Immunohistochemistry analysis of paraffin-embedded human colon using PKR (GB111096) at dilution of 1: 1200 |
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Immunohistochemistry analysis of paraffin-embedded mouse brain using PKR (GB111096) at dilution of 1: 1200 |
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Immunohistochemistry analysis of paraffin-embedded mouse spleen using PKR (GB111096) at dilution of 1: 1200 |
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Immunohistochemistry analysis of paraffin-embedded mouse heart using PKR (GB111096) at dilution of 1: 1200 |
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Immunohistochemistry analysis of paraffin-embedded mouse pneumonia using PKR (GB111096) at dilution of 1: 1200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
