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Anti-POLR2B Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | POLR2B rabbit polyclonal |
Gene Name: | DNA-directed RNA polymerase II subunit RPB2 |
Synonyms: | hRPB140, hsRPB2, POL2RB, POLR2B, RNA polymerase II subunit 2, RNA polymerase II subunit B2, RPB2 |
Immunogen: | Recombinant protein corresponding to Mouse POLR2B |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 134 kDa |
Observed MW. | 140 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 300-1: 600 | U-251MG, HEK293 |
Background
POLR2B belongs to the RNA polymerase beta chain family. It is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is the second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. POLR2B proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. The antibody is specific to POLR2B.
Images
 | Western blot analysis of POLR2B (GB113255) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
