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Recombinant Anti-Phospho-AKT (Ser473) Rabbit mAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Gene Name: | RAC-beta serine/threonine-protein kinase， RAC-gamma serine/threonine-protein kinase， RAC-alpha serine/threonine-protein kinase |
Synonyms: | AKT, AKT (pan), AKT pan, AKT1, AKT1/2/3, pan AKT, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha, total AKT |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human AKT(Ser473) |
Uniprot ID: | P31751, Q9Y243, P31749 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 56 kDa / 60 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | HeLa,NIH3T3,C6 |
Background
An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK.These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr.Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation.While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery.
Images
| WB analysis of Phospho-AKT(Ser473)(GB150002). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
