Recombinant Anti-Phospho-IKB alpha (S32) Rabbit mAb (100 μl)

NF-kappa-B inhibitor alpha

Synonyms:

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, Ikβ-α, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

Immunogen:

KLH conjugated Synthetic phosphopeptide corresponding to Human IκB alpha（S32）

Uniprot ID:

P25963

Isotype:

IgG

Purity:

Affinity purification

Predicted MW. / Observed MW.

36 kDa / 36 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

HeLa, NIH3T3, C6

WB analysis of Phospho-IKB alpha（S32）(GB15212).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

WB analysis of Phospho-IKB alpha（S32）(GB15212).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

WB analysis of Phospho-IKB alpha（S32）(GB15212).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.