Recombinant Anti-RFP Mouse mAb (100 μl)

Recombinant Anti-RFP Mouse mAb (100 μl)

Brand: ServiceBio
Cat. #: GB155054-100
Availability: In Stock
$259.00
-+
Reactivity:     
Applications: WB    
Host Species: Mouse    
Clonality: Monoclonal    
Gene Name:

Red fluorescent protein drFP583

Synonyms:

Red fluorescent protein, Red fluorescent protein eqFP611, RFPTag, RFP-Tag, RFP Tag

Immunogen:

Recombinant protein corresponding to RFP

Uniprot ID:

Q9U6Y8

Isotype:

IgG1

Purity:

Affinity purification

Predicted MW. / Observed MW.

26 kDa / 32 kDa




Product Usage Information

Applications

Species

Dilution

Positive Tissue

WB

Species independent

1: 500-1: 1000

RFP recombinant protein




Background

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Red Fluorescent Protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.




Images

01 GB155054_RFP_A16_ZCM23038240315A_d1000_WB_WM.png

WB analysis of RFP(GB155054).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h.




Storage

Storage             Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


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