Reactivity: | M,R |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | RPB3 rabbit polyclonal |
Gene Name: | DNA-directed RNA polymerase II subunit RPB3 |
Synonyms: | hRPB33, hsRPB3, POLR2C, RNA polymerase II subunit 3, RNA polymerase II subunit B3, RPB3, RPB31, RPB33 |
Immunogen: | Recombinant protein corresponding to Mouse RPB3 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 31 kDa |
Observed MW. | 31 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse | 1: 300-1: 600 | NIH/3T3 |
Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft .
Images
Western blot analysis of RPB3 (GB113315) at dilution of 1: 600 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |