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Anti-RPL10 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 60S ribosomal protein L10 |
Synonyms: | Protein QM homolog, Ribosomal protein L10, Rpl10, Qm, DXS648, L10, NOV, Tumor suppressor QM, Wilms tumor related protein, Protein QM, Laminin receptor homolog |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse RPL10 |
Uniprot ID: | Q6ZWV3 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 25 kDa / 25 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 2000-1: 4000 | pancreas |
IHC/IF | Human, Mouse, Rat | 1: 2000-1: 4000 | tonsil, brain, kidney |
ICC/IF | Human, Mouse, Rat | 1: 100-1: 400 | HeLa,NIH-3T3 |
Background
This gene encodes a ribosomal protein that is a component of the 60S ribosome subunit. The related protein in chicken can bind to c-Jun and can repress c-Jun-mediated transcriptional activation. Some studies have detected an association between variation in this gene and autism spectrum disorders, though others do not detect this relationship. There are multiple pseudogenes of this gene dispersed throughout the genome. Alternative splicing results in multiple transcript variants.
Images
| Western blot analysis of RPL10 (GB113267) at dilution of 1: 4000 Lane 1: C6 cell lysate Lane 2: Jurkat cell lysate Lane 3: Mouse pancreas tissue lysate Lane 4: Rat pancreas tissue lysate |
| Western blot analysis of RPL10 (GB113267) at dilution of 1: 4000 Lane 1: NIH-3T3 cell lysate Lane 2: PC-12 cell lysate Lane 3: Mouse pancreas tissue lysate Lane 4: Rat pancreas tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded human tonsil using RPL10 (GB113267) at dilution of 1: 4000 |
| Immunohistochemistry analysis of paraffin-embedded mouse brain using RPL10 (GB113267) at dilution of 1: 4000 |
| Immunohistochemistry analysis of paraffin-embedded rat brain using RPL10 (GB113267) at dilution of 1: 4000 |
| Immunohistochemistry analysis of paraffin-embedded mouse kidney using RPL10 (GB113267) at dilution of 1: 4000 |
| Immunohistochemistry analysis of paraffin-embedded mouse pancreas using RPL10 (GB113267) at dilution of 1: 4000 |
| Immunohistochemistry analysis of paraffin-embedded mouse rectum using RPL10 (GB113267) at dilution of 1: 4000 |
| Immunohistochemistry analysis of paraffin-embedded rat rectum using RPL10 (GB113267) at dilution of 1: 4000 |
| ICC analysis of RPL10(GB113267)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| ICC analysis of RPL10(GB113267)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
