Reactivity: | H,M & H & M |
Applications: | WB & IHC/IF & ICC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | 60S acidic ribosomal protein P1 |
Synonyms: | P1, ribosomal protein, large, P1, RPLP1, RPP1, RRP1 |
Immunogen: | Recombinant protein corresponding to Mouse RPLP1 |
Uniprot ID: | P47955 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. / Observed MW. | 11 kDa / 18 kDa |
Product Usage Information
WB | Human, Mouse | 1: 300-1: 500 | brain |
IHC/IF | Human | 1: 700-1: 1400 | esophageal carcinoma, gastric carcinoma |
ICC/IF | Mouse | 1: 100-1: 400 | NIH-3T3 |
Background
Plays an important role in the elongation step of protein synthesis.
Images
Western blot analysis of RPLP1 (GB114437) at dilution of 1: 500 | |
Immunohistochemistry analysis of paraffin embedded human esophageal carcinoma using RPLP1 (GB114437) at dilution of 1: 700 | |
Immunohistochemistry analysis of paraffin embedded human gastric carcinoma using RPLP1 (GB114437) at dilution of 1: 700 | |
Immunofluorescence analysis of paraffin embedded human colon cancer using RPLP1 (GB114437) at dilution of 1: 700 | |
ICC analysis of RPLP1(GB114437)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |