Reactivity: | H,M,R & H |
Applications: | WB & ICC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | 40S ribosomal protein S4, Y isoform 1 |
Synonyms: | Small ribosomal subunit protein eS4, RPS4Y1, RPS4Y, PRO2646 |
Immunogen: | Recombinant protein corresponding to Mouse RPS4Y1 |
Uniprot ID: | P22090 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 29 kDa / 29 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | Stomach, ovary, testis |
ICC/IF | Human | 1: 100-1: 400 | HeLa |
Background
RPS4Y1, a Y-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). RPS4Y maps to a 90-kb segment that has been implicated in Turner syndrome. As a homolog with RPS4X, the antibody can recognize endogenous level of RPS4Y and RPS4X, at 29-32kDa.
Images
Western blot analysis of RPS4Y1 (GB112983) at dilution of 1: 2000 | |
ICC analysis of RPS4Y1(GB112983)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |