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Anti-RSUME Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | RWD domain-containing protein 3 |
Synonyms: | RSUME, RWD domain containing 3, RWDD3 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse RSUME |
Uniprot ID: | Q8VIL2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Product Usage Information
IHC/IF | Mouse, Rat | 1: 400-1: 1200 | colon, pancreas, brain |
Background
Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Positively regulates the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Negatively regulates the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Has no effect on ubiquitination.
Images
| Immunohistochemistry analysis of paraffin embedded rat colon using RWDD3 (GB114841) at dilution of 1: 400 |
| Immunohistochemistry analysis of paraffin embedded mouse pancreas using RWDD3 (GB114841) at dilution of 1: 400 |
| Immunohistochemistry analysis of paraffin embedded mouse brain using RWDD3 (GB114841) at dilution of 1: 400 |
| Immunohistochemistry analysis of paraffin embedded rat brain using RWDD3 (GB114841) at dilution of 1: 400 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
