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Anti-S Tag Rabbit pAb (100 μl)
| Reactivity: | |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | S Tag |
Synonyms: | KETAAAKFERQHMDS tag, S peptide epitope tag, S peptide, S15, S-Tag, S-tag, S TAG |
Immunogen: | KLH conjugated Synthetic peptide corresponding to KETAAAKFERQHMDS |
Isotype: | IgG |
Purity: | Affinity purification |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Tag (All species) | 1: 500-1: 1000 | S-Tag fusion protein |
Special note
Epitope labeling has been proved to be an effective method that can be performed on target proteins. Tag antibody can be used in a variety of applications, including ChIP, CoIP, ELISA, FC, IF, IHC, IP, WB and so on.
Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The S tag is corresponds to amino acid residues KETAAAKFERQHMDS of pancreatic ribonuclease A (RNase A). Many recombinant proteins have been engineered to express the S tag. It is believed that the peptide with its abundance of charged and polar residues could improve solubility of proteins it is attached to. Moreover, the peptide alone is thought not to fold into a distinct structure.
This tag facilitates the detection, isolation, and purification of the proteins.
Images
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Western blot analysis of S-Tag (GB111252) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
