Recombinant Anti-S100 beta Mouse mAb (100 μl)

Protein S100-B

Synonyms:

S100B, NEF, S100, S100-B, S100beta,S100 calcium binding protein B, S100β

Immunogen:

KLH conjugated Synthetic peptide corresponding to Mouse S100B

Uniprot ID:

P50114

Isotype:

lgG1

Purity:

Affinity purification

Subcellular location:

Nucleus, Cytoplasm, Secreted

IHC/IF

Human, Mouse, Rat

1: 500-1: 1000

glioblastoma multiforme, brain

IHC analysis of S100B (GB15359).
Sample: Human glioblastoma multiforme (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.

IHC analysis of S100B (GB15359).
Sample: Mouse Brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.

IHC analysis of S100B (GB15359).
Sample: Rat Brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.