Recombinant Anti-S100 beta Mouse mAb (100 μl)

Recombinant Anti-S100 beta Mouse mAb (100 μl)

Brand: ServiceBio
Cat. #: GB15360-100
Availability: In Stock
$259.00
-+
Reactivity: H,M,R    
Applications: IHC/IF    
Host Species: Mouse    
Clonality: Monoclonal    
Gene Name:

Protein S100-B

Synonyms:

S100B, NEF, S100, S100-B, S100beta, S100 calcium binding protein B, S100β

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human S100B

Uniprot ID:

P04271

Isotype:

IgG1

Purity:

Affinity purification

Subcellular location:

Nucleus, Cytoplasm,Secreted




Product Usage Information

IHC/IF

Human, Mouse, Rat

1: 500-1: 1000

Ganglion, brain




Background

S100B is glial-specific and is expressed primarily by astrocytes, but not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells.This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly.




Images

01 GB15360_S100 beta_ZSM21120_ZSM21120231208_d1000_H ganglion_40.0x_IHC_WM.png


IHC analysis of S100 beta(GB15360).
Sample: Human ganglion, 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH9.0)(G1203)), 98°C, 20 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

02 GB15360_S100 beta_ZSM21120_ZSM21120231208_d1000_M brain_40.0x_IHC_WM.png

IHC analysis of S100 beta(GB15360).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH9.0)(G1203)), 98°C, 20 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

03 GB15360_S100 beta_ZSM21120_ZSM21120231208_d1000_R brain_40.0x_IHC_WM.png

IHC analysis of S100 beta(GB15360).
Sample: Rat brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH9.0)(G1203)), 98°C, 20 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.




Storage

Storage             Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


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