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| Reactivity: | M |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Sterol regulatory element-binding protein cleavage-activating protein |
Synonyms: | SREBF chaperone, SCAP, KIAA0199, SREBF chaperone protein |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse SCAP |
Uniprot ID: | Q6GQT6 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 140 kDa / 130 kDa |
Product Usage Information
WB | Mouse | 1: 300-1: 600 | brain |
Background
Escort protein required for cholesterol as well as lipid homeostasis . Regulates export of the SCAP-SREBP complex from the endoplasmic reticulum to the Golgi upon low cholesterol, thereby regulating the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 . At high sterol concentrations, formation of a ternary complex with INSIG (INSIG1 or INSIG2) leads to mask the ER export signal in SCAP, promoting retention of the complex in the endoplasmic reticulum . Low sterol concentrations trigger release of INSIG, a conformational change in the SSD domain of SCAP, unmasking of the ER export signal, promoting recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi: in the Golgi, SREBPs are then processed, releasing the transcription factor fragment of SREBPs from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway . Binds cholesterol via its SSD domain .
Images
| Western blot analysis of SCAP (GB114675) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
