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Anti-SHARPIN Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Sharpin |
Synonyms: | hSIPL1, SHARPIN, SIPL1 |
Immunogen: | Recombinant protein corresponding to Mouse SHARPIN |
Uniprot ID: | Q91WA6 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 40 kDa / 38 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 500-1: 1000 | skeletal muscle |
Background
Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex.
Images
| Western blot analysis of SHARPIN (GB114444) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
