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| Reactivity: | M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Spindle and kinetochore-associated protein 2 |
Synonyms: | FAM33A, FLJ12758, Spindle and KT associated 2 |
Immunogen: | Recombinant protein corresponding to Mouse SKA2 |
Uniprot ID: | Q9CR46 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cytoskeleton |
Predicted MW. / Observed MW. | 14 kDa / 15 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 1000-1: 2000 | heart |
IHC/IF | Human, Mouse, Rat | 1: 1000-1: 3000 | liver cancer, kidney, lung, colon |
Background
Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner.
Images
| Western blot analysis of SKA2 (GB114559) at dilution of 1: 1000 |
| Immunohistochemistry of paraffin embedded human Liver cancer using SKA2 (GB114559) at dilution of 1: 3000 |
| Immunohistochemistry of paraffin embedded mouse kidney using SKA2 (GB114559) at dilution of 1: 3000 |
| Immunofluorescence of paraffin embedded mouse lung using SKA2 (GB114559) at dilution of 1: 900 |
| Immunofluorescence of paraffin embedded rat colon using SKA2 (GB114559) at dilution of 1: 900 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
