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Anti-SQSTM1 / p62 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Sequestosome-1 |
Synonyms: | SQSTM1, A170, OSIL, PDB3, ZIP3, p60, p62, p62B, FTDALS3, Sequestosome 1, NADGP, DMRV |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human SQSTM1 |
Uniprot ID: | Q13501 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 48 kDa / 62 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | HeLa, HIN3T3, HepG2 |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | ovarian cancer, lung cancer |
ICC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | HeLa, NIH/3T3 |
Background
The product of SQSTM1 gene, Sequestosome-1, also kown as ubiquitin-binding protein p62, is an autophagosome cargo protein that targets other proteins that bind to it for selective autophagy. By interacting with GATA4 and targeting it for degradation, it can inhibit GATA-4 associated senescence and senescence-associated secretory phenotype.
Images
Lane 1: HeLa (untreated) cell lysate
Lane 2: HeLa (treated with Chloroquine, 50 μM, overnight ) cell lysate
Lane 3: NIH3T3 (untreated) cell lysate
Lane 4: NIH3T3 (treated with Chloroquine, 50 μM, overnight ) cell lysate
Lane 5: HepG2 cell lysate
Lane 6: MCF7 cell lysate
Lane 7: A549 cell lysate
Lane 8: L929 cell lysate
Lane 9: PC-12 cell lysate
| |
 | Immunohistochemistry analysis of paraffin-embedded human ovarian cancer using P62 (GB11531) at dilution of 1: 500 |
 | Immunohistochemistry analysis of paraffin-embedded human lung cancer using P62 (GB11531) at dilution of 1: 500 |
 | Immunocytochemistry analysis of 4% paraformaldehyde-fixed Hela cell untreatd using P62 (GB11531) at dilution of 1: 500 |
 | Immunocytochemistry analysis of 4% paraformaldehyde-fixed Hela cell with chloroquine treated using P62 (GB11531) at dilution of 1: 500 |
 | Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH/3T3 cell untreatd using P62 (GB11531) at dilution of 1: 500 |
 | Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH/3T3 cell with chloroquine treated using P62 (GB11531) at dilution of 1: 500 |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
