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Recombinant Anti-Sarcomeric Alpha Actinin Mouse mAb (100 μl)
| Reactivity: | M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Alpha-actinin-2 |
Synonyms: | ACTN2, CMD1AA, CMH23, Actinin alpha 2, alpha Actinin 2,Actn2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse alpha Actinin 2 |
Uniprot ID: | Q9JI91 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 104 kDa / 104 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Mouse, Rat | 1: 1000-1: 3000 | heart, skeletal muscle |
IHC/IF | Human, Mouse, Rat | 1: 400-1: 800 | kindey, heart |
Background
Alpha-actinin 2 is a protein which in humans is encoded by the ACTN2 gene. This gene encodes an alpha-actinin isoform that is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs. The primary function of alpha-actinin 2 is to crosslink filamentous actin molecules and titin molecules from adjoining sarcomeres at Z-discs, a function that is modulated by phospholipids. Alpha-actinin 2 also functions in docking signalling molecules at Z-discs, and additional studies have also implicated alpha-actinin 2 in the binding of cardiac ion channels, Kv1.5 in particular.
Images
| WB analysis of Sarcomeric Alpha Actinin（GB15555). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 3000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of Sarcomeric Alpha Actinin(GB15555). Sample: Human kindey, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L),Ready to use(G1301), RT, 20min. |
| IHC analysis of Sarcomeric Alpha Actinin(GB15555). Sample: Mouse heart, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L),Ready to use(G1301), RT, 20min. |
| IHC analysis of Sarcomeric Alpha Actinin(GB15555). Sample: Mouse kindey, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L),Ready to use(G1301), RT, 20min. |
| IHC analysis of Sarcomeric Alpha Actinin(GB15555). Sample: Rat heart, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L),Ready to use(G1301), RT, 20min. |
| IHC analysis of Sarcomeric Alpha Actinin(GB15555). Sample: Rat kindey, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L),Ready to use(G1301), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
