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Anti-Securin Rabbit pAb (100 μl)
| Reactivity: | M,R & H,M,R & M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Securin |
Synonyms: | Pituitary tumor-transforming gene 1 protein, Pttg1, Pttg, Cut2, EAP1, ESP1, TUTR1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Securin |
Uniprot ID: | Q9CQJ7 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 22 kDa / 26 kDa |
Images
WB | Mouse, Rat | 1: 500-1: 1000 | testis, cecum, skin |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 2800 | placenta, testis |
ICC/IF | Mouse, Rat | 1: 50-1: 300 | NIH-3T3 |
Background
PTTG1 is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The protein has transforming activity in vitro and tumorigenic activity in vivo, and is highly expressed in various tumors. This protein contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression.
Images
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Western blot analysis of Securin (GB111047) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded mouse placenta using Securin (GB111047) at dilution of 1: 1400 (400x lens) |
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Immunohistochemistry of paraffin embedded rat testis using Securin (GB111047) at dilution of 1: 1400 (400x lens) |
| ICC analysis of Securin(GB111047)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Primary antibody: 1: 50, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |