Your shopping cart is empty!
| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Mothers against decapentaplegic homolog 2 |
Synonyms: | SMAD2, JV18, V18-1, MADH2, MADR2, hMAD-2, hSMAD family member 2, Mothers against DPP homolog 2, hSMAD2 |
Immunogen: | Recombinant protein corresponding to Mouse Smad2 |
Uniprot ID: | Q62432 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 49-52 kDa / 49-60 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | heart, liver, brain |
IHC/IF | Mouse, Rat | 1: 1000-1: 2000 | heart, skeletal muscle, placenta, skeletal muscle, muscle, inflammatory heart |
Background
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Images
|
Western blot analysis of Smad2 (GB11511) at dilution of 1:800. |
 |
Immunohistochemistry analysis of paraffin-embedded mouse heart using Smad2 (GB11511) at dilution of 1:1000. |
|
Immunohistochemistry analysis of paraffin-embedded rat skeletal muscle using Smad2 (GB11511) at dilution of 1:1000. |
|
Immunofluorescent analysis of paraformaldehyde-fixed mouse placenta using smad2 (GB11511) at dilution of 1:500. |
|
Immunofluorescent analysis of paraformaldehyde-fixed mouse skeletal muscle using smad2 (GB11511) at dilution of 1:1000. |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse muscle using smad2 (GB11511) at dilution of 1:500. |
|
Immunofluorescent analysis of paraformaldehyde-fixed rat inflammatory heart using smad2 (GB11511) at dilution of 1:500. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
