Reactivity: | M,R |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | TAF5 rabbit polyclonal |
Gene Name: | Transcription initiation factor TFIID subunit 5 |
Synonyms: | Transcription initiation factor TFIID 100 kDa subunit, TAF(II)100, TAFII-100, TAFII100, Taf5, p85, TAF2D, TAFII 80 |
Immunogen: | Recombinant protein corresponding to Mouse TAF5 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 87 kDa |
Observed MW. | 100 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse, Rat | 1: 500-1: 800 | testis, liver |
Background
TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with the histone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stable complex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions of TAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55, also have been observed.
Images
Western blot analysis of TAF5 (GB113386) at dilution of 1: 800 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |