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Anti-TATA binding protein TBP Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | TATA binding protein TBP rabbit polyclonal antibody |
Gene Name: | TATA-box-binding protein |
Synonyms: | GTF2D, GTF2D1, SCA17, TATA-binding factor, TATA-box factor, TATA sequence binding protein, TBP, TF2D, TFIID |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse TBP |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 35 kDa |
Observed MW. | 35 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 200-1: 500 | testis, ovary |
Background
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.
Images
 | Western blot analysis of TBP (GB113810) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
