Reactivity: | M,R & H |
Applications: | WB & IHC |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | TGT rabbit polyclonal |
Gene Name: | Queuine tRNA-ribosyltransferase catalytic subunit 1 |
Synonyms: | FP3235, Guanine insertion enzyme, QTRT1, TGT, TGUT, tRNA guanine transglycosylase |
Immunogen: | Recombinant protein corresponding to Mouse TGT |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 44 kDa |
Observed MW. | 44 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse, Rat | 1: 500-1: 800 | brain |
IHC | Human | 1: 3200-1: 6400 | colon cancer, colon |
Background
This gene encodes the catalytic subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine and tyrosine. A pseudogene of this gene is located on the long arm of chromosome X.
Images
Western blot analysis of TGT (GB113197) at dilution of 1: 800 | |
Immunohistochemistry analysis of paraffin-embedded human colon cancer using TGT (GB113197) at dilution of 1: 6400 | |
Immunohistochemistry analysis of paraffin-embedded human colon using TGT (GB113197) at dilution of 1: 6400 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |