Your shopping cart is empty!
Anti-TNF-alpha Rabbit pAb (100 μl)
| Reactivity: | M |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | TNF alpha rabbit polyclonal antibody |
Gene Name: | tumor necrosis factor (TNF superfamily, member 2) |
Synonyms: | Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor |
Immunogen: | Recombinant protein corresponding to Mouse TNF alpha |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 30 kDa |
Observed MW. | 25/28 kDa |
Uniprot ID: | P06804 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse | 1: 500-1: 1500 | RAW264.7 |
Background
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
Images
 | Western blot analysis of TNF alpha (GB113968) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
