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Anti-TYW1B Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | TYW1B rabbit polyclonal antibody |
Gene Name: | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1B |
Synonyms: | Radical S-adenosyl methionine and flavodoxin domain-containing protein 2, TYW1B, RSAFD2, tRNA wybutosine-synthesizing protein 1 homolog B |
Immunogen: | Recombinant protein corresponding to Human TYW1B |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Secreted |
Predicted MW. | 77 kDa |
Observed MW. | 34 kDa |
Uniprot ID: | Q6NUM6 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 3000-1: 6000 | brain, liver |
IHC/IF | Human, Mouse, Rat | 1: 600-1: 2000 | brain, breast cancer, epencephalon, heart |
Background
Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis .
Images
| Western blot analysis of TYW1B (GB112605) at dilution of 1: 3000 |
| Immunohistochemistry of paraffin embedded human breast cancer using TYW1B (GB112605) at dilution of 1: 700 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse brain using TYW1B (GB112605) at dilution of 1: 700 (400x lens) |
| Immunohistochemistry of paraffin embedded rat brain using TYW1B (GB112605) at dilution of 1: 700 (400x lens) |
| Immunofluorescence of paraffin embedded mouse brain using TYW1B (GB112605) at dilution of 1: 650 (400x lens) |
| Immunofluorescence of paraffin embedded mouse heart using TYW1B (GB112605) at dilution of 1: 650 (400x lens) |
| Immunofluorescence of paraffin embedded rat epencephalon using TYW1B (GB112605) at dilution of 1: 650 (400x lens) |
| Immunofluorescence of paraffin embedded rat heart using TYW1B (GB112605) at dilution of 1: 650 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
