Anti-UCP1 Rabbit pAb (100 μl)

UCP1 rabbit polyclonal antibody

Gene Name:

Mitochondrial brown fat uncoupling protein 1

Synonyms:

UCP1, Solute carrier family 25 member 7, Thermogenin, Slc25a7

Immunogen:

KLH conjugated Synthetic peptide corresponding to Mouse UCP1

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Mitochondrion

Predicted MW.

33 kDa

Observed MW.

33 kDa

Uniprot ID:

P12242, P04633

WB

Mouse, Rat

1: 500-1: 1000

brown fat

IHC/IF

Mouse, Rat

1: 200-1: 600

brown adipose tissue

WB analysis of UCP1 (GB112174).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

IHC analysis of UCP1 (GB112174).
Sample: Mouse brown fat (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 300, 4°C overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

IHC analysis of UCP1 (GB112174).
Sample: Rat brown fat (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 300, 4°C overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.