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Anti-Ube2N / Ubc13 Mouse mAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Ubiquitin-conjugating enzyme E2 N |
Synonyms: | Ube2n, Bendless like ubiquitin conjugating enzyme, BLU, UBC 13, Ube2n, Ubiquitin carrier protein N, Ubiquitin conjugating enzyme E2N |
Immunogen: | Recombinant protein corresponding to Mouse Ubc13 |
Uniprot ID: | P61089 |
Isotype: | IgG2b,κ |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 17 kDa / 17 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | brain, spinal cord, thymus |
Background
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
Images
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Western blot analysis of UBC13 (GB12986) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
