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Recombinant Anti-alpha Tubulin antibody (HRP Conjugated) (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | |
| Clonality: | |
| Gene Name: | Tubulin alpha-1A chain |
Synonyms: | TUBA1A, B-ALPHA-1, LIS3, TUBA3, tubulin alpha 1a,TUBA1B, K-ALPHA-1, tubulin alpha 1b |
Immunogen: | Recombinant protein corresponding to Mouse alpha Tubulin |
Uniprot ID: | P68369 |
Isotype: | IgG1 |
Conjugation | HRP |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 50 kDa / 55 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 3000-1: 6000 | HeLa, Hep G2, lung |
Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-alpha Tubulin antibody(ZB15200-HRP) for 3-4 hours at room temperature (20-25°C) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Images
| WB analysis of alpha Tubulin(ZB15200-HRP). Antibody: HRP conjugated anti-alpha Tubulin antibody 1: 3000, 4°C overnight. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
